CTNNBL1 monoclonal antibody, clone 1E4F5
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell lysate; Lane 2: Jurkat cell lysate; Lane 3: HEK293 cell lysate; Lane 4: A431 cell lysate; Lane 5: HepG2 cell lysate; Lane 6: RAJI cell lysate with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CTNNBL1 (AA: 390-557)-hIgGFc transfected HEK293 cells] with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human CTNNBL1 (AA: 390-557) recombinant protein (Expected MW is 45.8 kDa) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon cancer tissues (A) and esophageal cancer tissues (B) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of Hela cells with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human CTNNBL1.
Immunogen
Recombinant protein corresponding to amino acids 390-557 of human CTNNBL1.
Host
Mouse
Theoretical MW (kDa)
65.2
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunofluorescence (1:200-1:1000)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Hela cell lysate; Lane 2: Jurkat cell lysate; Lane 3: HEK293 cell lysate; Lane 4: A431 cell lysate; Lane 5: HepG2 cell lysate; Lane 6: RAJI cell lysate with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [CTNNBL1 (AA: 390-557)-hIgGFc transfected HEK293 cells] with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human CTNNBL1 (AA: 390-557) recombinant protein (Expected MW is 45.8 kDa) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human colon cancer tissues (A) and esophageal cancer tissues (B) with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.Immunofluorescence
Immunofluorescent staining of MCF-7 cells with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of Hela cells with CTNNBL1 monoclonal antibody, clone 1E4F5 (Cat# MAB12247) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — CTNNBL1
Entrez GeneID
56259Gene Name
CTNNBL1
Gene Alias
C20orf33, FLJ21108, NAP, NYD-SP19, P14L, PP8304, dJ633O20.1
Gene Description
catenin, beta like 1
Omim ID
611537Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains an acidic domain, a putative bipartite nuclear localization signal, a nuclear export signal, a leucine-isoleucine zipper, and phosphorylation motifs. In addition, the encoded protein contains Armadillo/beta-catenin-like repeats, which have been implicated in protein-protein interactions. Although the function of this protein has not been determined, the C-terminal portion of the protein has been shown to possess apoptosis-inducing activity. [provided by RefSeq
Other Designations
beta catenin-like 1|nuclear associated protein
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Interactome
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Disease
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