FTL monoclonal antibody, clone 6E10E4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate; Lane 2: K562 cell lysate with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:500-1:2000 dilution.
Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [FTL (AA: 1-175)-hIgGFc transfected HEK293 cells] with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:500-1:2000 dilution.
Western Blot (Recombinant protein)
Western blot analysis of human FTL (AA: 1-175) recombinant protein (Expected MW is 45.5 kDa) with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:500-1:2000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human ovarian cancer tissues (A) and esophageal tissues (B) with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:200-1:1000 dilution.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:10000 dilution.
Flow Cytometry
Flow cytometric analysis of HepG2 cells with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:200-1:400 dilution (Green) and negative control (Red).
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human FTL.
Immunogen
Recombinant protein corresponding to full length human FTL.
Host
Mouse
Theoretical MW (kDa)
20
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: HepG2 cell lysate; Lane 2: K562 cell lysate with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:500-1:2000 dilution.Western Blot (Transfected lysate)
Western blot analysis of Lane 1: Negative control [HEK293 cell lysate]; Lane 2: Over-expression lysate [FTL (AA: 1-175)-hIgGFc transfected HEK293 cells] with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:500-1:2000 dilution.Western Blot (Recombinant protein)
Western blot analysis of human FTL (AA: 1-175) recombinant protein (Expected MW is 45.5 kDa) with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:500-1:2000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human ovarian cancer tissues (A) and esophageal tissues (B) with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:200-1:1000 dilution.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:10000 dilution.Flow Cytometry
Flow cytometric analysis of HepG2 cells with FTL monoclonal antibody, clone 6E10E4 (Cat# MAB12245) at 1:200-1:400 dilution (Green) and negative control (Red). -
Gene Info — FTL
Entrez GeneID
2512Gene Name
FTL
Gene Alias
MGC71996
Gene Description
ferritin, light polypeptide
Gene Ontology
HyperlinkGene Summary
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq
Other Designations
L apoferritin|ferritin L subunit|ferritin L-chain|ferritin light chain|ferritin light polypeptide-like 3
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Interactome
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Disease
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