HLA-G monoclonal antibody, clone 5A6G7
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More Files
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Western Blot (Transfected lysate)
Western blotting analysis of whole cell lysate of HLA - G stable transfectants (various splice variants) using HLA - G monoclonal antibody, clone 5A6G7 (Cat # MAB0965) . Lane 1 : M8 cell line transfected with empty vector. Lane 2 : M8 cell line transfected with HLA - G1. Lane 3 : M8 cell line transfected with HLA - G5. Lane 4 : M8 cell line transfected with HLA - G6.
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Specification
Product Description
Mouse monoclonal antibody raised against synthetic peptide of HLA-G.
Immunogen
A synthetic peptide (conjugated with OVA) corresponding to C-terminus of human soluble HLA-G5 and HLA-G6.
Host
Mouse
Reactivity
Human
Specificity
This antibody allows to discriminate between soluble HLA-G protein yielded by shedding from membrane-bound HLA-G forms that do not contain the intron 4-encoded epitope and soluble HLA-G5/HLA-G6 produced from intron 4-retaining alternatively spliced mRNAs.
Form
Liquid
Isotype
IgG1
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4 (0.09% sodium azide)
Storage Instruction
Store at 4°C. Do not freeze.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blotting analysis of whole cell lysate of HLA - G stable transfectants (various splice variants) using HLA - G monoclonal antibody, clone 5A6G7 (Cat # MAB0965) . Lane 1 : M8 cell line transfected with empty vector. Lane 2 : M8 cell line transfected with HLA - G1. Lane 3 : M8 cell line transfected with HLA - G5. Lane 4 : M8 cell line transfected with HLA - G6.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — HLA-G
Entrez GeneID
3135Gene Name
HLA-G
Gene Alias
MHC-G
Gene Description
major histocompatibility complex, class I, G
Gene Ontology
HyperlinkGene Summary
HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq
Other Designations
HLA class I molecule|HLA-G histocompatibility antigen, class I, G|MHC class I antigen|OTTHUMP00000039485|b2 microglobulin
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Interactome
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Pathway
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Disease
- Abortion
- Anemia
- Arthritis
- Asthma
- Autoimmune Diseases
- Behcet Syndrome
- Birth Weight
- Bronchial Hyperreactivity
- Carcinoma
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