FOXM1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FOXM1 full length
Host
Human
Theoretical MW (kDa)
84
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FOXM1 antibody (H00002305-M01) by Western Blots.
SDS-PAGE Gel
FOXM1 transfected lysate
Western Blot
Lane 1: FOXM1 transfected lysate ( 84 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — FOXM1
Entrez GeneID
2305GeneBank Accession#
NM_202002Protein Accession#
NP_973731Gene Name
FOXM1
Gene Alias
FKHL16, FOXM1B, HFH-11, HFH11, HNF-3, INS-1, MPHOSPH2, MPP-2, MPP2, PIG29, TGT3, TRIDENT
Gene Description
forkhead box M1
Omim ID
602341Gene Ontology
HyperlinkGene Summary
drosophila
Other Designations
Forkhead, drosophila, homolog-like 16|M-phase phosphoprotein 2|forkhead-like 16
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