FXR1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-FXR1 full length
Host
Human
Theoretical MW (kDa)
61
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-FXR1 antibody (H00008087-M01) by Western Blots.
SDS-PAGE Gel
FXR1 transfected lysate
Western Blot
Lane 1: FXR1 transfected lysate ( 61 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — FXR1
Entrez GeneID
8087GeneBank Accession#
BC028983Protein Accession#
AAH28983Gene Name
FXR1
Gene Alias
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Gene Description
fragile X mental retardation, autosomal homolog 1
Omim ID
600819Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an RNA binding protein that interacts with the functionally-similar proteins FMR1 and FXR2. These proteins shuttle between the nucleus and cytoplasm and associate with polyribosomes, predominantly with the 60S ribosomal subunit. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
fragile X mental retardation-related protein 1
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