FFPE FISH PreTreatment Kit 1
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Specifications
Product Description
Pre-treatment is an essential step for effective FISH on Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections. This kit contains key reagents used to pretreat FFPE tissue sections from dewaxing with xylene through proteolytic digestion to help permeabilization of cell membranes to facilitate penetration of fluorescence labeled probes. After proteolytic digestion, proceed with subsequent steps with the FISH assay protocol.
Regulation Status
For research use only (RUO)
Supplied Product
Kit content:
1. 1x Paraffin Pretreatment Solution, 500 mL
2. Protease (Pepsin), 0.5 mL x 10 vialsStorage Instruction
After arrival, please store the Paraffin Pretreatment Solution at room temperature (RT), and store the Protease (Pepsin) at -20°C.
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Applications
Fluorescent In Situ Hybridization (Cell)
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Publication Reference
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Syndromic MEN1 parathyroid adenomas consist of both subclonal nodules and clonally independent tumors.
Konstantin Bräutigam, Cédric Nesti, Philipp Riss, Christian Scheuba, Bruno Niederle, Tobias Grob, Annunziata Di Domenico, Maja Neuenschwander, Peter Mazal, Nastassja Köhn, Roman Trepp, Aurel Perren, Reto M Kaderli.
Virchows Archiv : an International Journal of Pathology 2024 Jan; [Epub].
Application：FISH, Human, parathyroid adenoma.
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Deficiency of PTEN and CDKN2A Tumor-Suppressor Genes in Conventional and Chondroid Chordomas: Molecular Characteristics and Clinical Relevance.
Chenlong Yang, Jianjun Sun, Lei Yong, Chen Liang, Tie Liu, Yulun Xu, Jun Yang, Xiaoguang Liu.
OncoTargets and Therapy 2020 May; 13:4649.
Application：FISH, Human, Chordoma.
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Clinical significance of PD-1/PD-Ls gene amplification and overexpression in patients with hepatocellular carcinoma.
Ma LJ, Feng FL, Dong LQ, Zhang Z, Duan M, Liu LZ, Shi JY, Yang LX, Wang ZC, Zhang S, Ding ZB, Ke AW, Cao Y, Zhang XM, Zhou J, Fan J, Wang XY, Gao Q.
Theranostics 2018 Nov; 8(20):5690.
Application：FISH, Human, Human hepatocellular carcinoma.
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FGF19 genetic amplification as a potential therapeutic target in lung squamous cell carcinomas.
Zhang X, Kong M, Zhang Z, Xu S, Yan F, Wei L, Zhou J.
Thoracic Cancer 2017 Sep; 8(6):655.
Application：FISH, Human, Human lung squamous cell carcinoma.
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Investigation of molecular alterations of AKT-3 in triple-negative breast cancer.
O'Hurley G, Daly E, O'Grady A, Cummins R, Quinn C, Flanagan L, Pierce A, Fan Y, Lynn MA, Rafferty M, Fitzgerald D, Pont幯 F, Duffy MJ, Jirström K, Kay EW, Gallagher WM.
Histopathology 2014 Apr; 64(5):660.
Application：FISH-P, Human, Breast cancer.
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Syndromic MEN1 parathyroid adenomas consist of both subclonal nodules and clonally independent tumors.
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