MAF1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
MAF1 transfected lysate.
Western Blot
Lane 1: MAF1 transfected lysate ( 28.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-MAF1 full-length
Host
Human
Theoretical MW (kDa)
28.9
Interspecies Antigen Sequence
Mouse (93); Rat (93)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-MAF1 antibody (H00084232-B01) by Western Blots.
SDS-PAGE Gel
MAF1 transfected lysate.
Western Blot
Lane 1: MAF1 transfected lysate ( 28.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — MAF1
Entrez GeneID
84232GeneBank Accession#
BC031273.1Protein Accession#
AAH31273.1Gene Name
MAF1
Gene Alias
MGC20332, MGC31779, MGC39758
Gene Description
MAF1 homolog (S. cerevisiae)
Omim ID
610210Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq
Other Designations
MAF1 protein|homolog of yeast MAF1
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