YY1 monoclonal antibody (M01), clone 2C4
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More Files
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant YY1.
Immunogen
YY1 (NP_003394, 221 a.a. ~ 320 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTH
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (100); Rat (100)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
YY1 monoclonal antibody (M01), clone 2C4 Western Blot analysis of YY1 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Transfected lysate)
Western Blot analysis of YY1 expression in transfected 293T cell line by YY1 monoclonal antibody (M01), clone 2C4.
Lane 1: YY1 transfected lysate(44.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — YY1
Entrez GeneID
7528GeneBank Accession#
NM_003403Protein Accession#
NP_003394Gene Name
YY1
Gene Alias
DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1
Gene Description
YY1 transcription factor
Omim ID
600013Gene Ontology
HyperlinkGene Summary
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. [provided by RefSeq
Other Designations
INO80 complex subunit S|Yin and Yang 1 protein
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Interactomes
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Diseases
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