SOD1 monoclonal antibody (M04), clone 10D5
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant SOD1.
Immunogen
SOD1 (AAH01034, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCITGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (83); Rat (82)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (42.68 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of SOD1 expression in transfected 293T cell line by SOD1 monoclonal antibody (M04), clone 10D5.
Lane 1: SOD1 transfected lysate (Predicted MW: 15.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged SOD1 is 0.1 ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to SOD1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — SOD1
Entrez GeneID
6647GeneBank Accession#
BC001034Protein Accession#
AAH01034Gene Name
SOD1
Gene Alias
ALS, ALS1, IPOA, SOD, homodimer
Gene Description
superoxide dismutase 1, soluble
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq
Other Designations
Cu /Zn superoxide dismutase|Cu/Zn superoxide dismutase|SOD, soluble|indophenoloxidase A|superoxide dismutase, cystolic
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Interactome
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Pathway
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Disease
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