PHYH monoclonal antibody (M01), clone 1F2-5B9

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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PHYH.
Immunogen
PHYH (AAH29512, 1 a.a. ~ 338 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MEQLRAAARLQIVLGHLGRPSAGAVVAHPTSGTISSASFHPQQFQYTLDNNVLTLEQRKFYEENGFLVIKNLVPDADIQRFRNEFEKICRKEVKPLGLTVMRDVTISKSEYAPSEKMITKVQDFQEDKELFRYCTLPEILKYVECFTGPNIMAMHTMLINKPPDSGKKTSRHPLHQDLHYFPFRPSDLIVCAWTAMEHISRNNGCLVVLPGTHKGSLKPHDYPKWEGGVNKMFHGIQDYEENKARVHLVMEKGDTVFFHPLLIHGSGQNKTQGFRKAISCHFASADCHYIDVKGTSQENIEKEVVGIAHKFFGAENSVNLKDIWMFRARLVKGERTNL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (78)
Isotype
IgG1 Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (62.92 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PHYH expression in transfected 293T cell line by PHYH monoclonal antibody (M01), clone 1F2-5B9.
Lane 1: PHYH transfected lysate(38.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PHYH is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — PHYH
Entrez GeneID
5264GeneBank Accession#
BC029512Protein Accession#
AAH29512Gene Name
PHYH
Gene Alias
LN1, LNAP1, PAHX, PHYH1, RD
Gene Description
phytanoyl-CoA 2-hydroxylase
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PhyH family and encodes a peroxisomal protein that is involved in the alpha-oxidation of 3-methyl branched fatty acids. Specifically, this protein converts phytanoyl-CoA to 2-hydroxyphytanoyl-CoA. Mutations in this gene have been associated with Refsum disease (RD) and deficient protein activity has been associated with Zellweger syndrome and rhizomelic chondrodysplasia punctata. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
Other Designations
phytanic acid oxidase|phytanoil-CoA alpha hydroxylase|phytanoyl-CoA 2 oxoglutarate dioxygenase|phytanoyl-CoA alpha-hydroxylase|phytanoyl-CoA dioxygenase, peroxisomal|phytanoyl-CoA hydroxylase (Refsum disease)
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Interactome
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Disease
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