AKT2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT2.
Immunogen
A synthetic peptide corresponding to amino acids 455-469 of human AKT2.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Specificity
This antibody only recognizes ~60 KDa of human AKT2, not AKT1 or AKT3. It also reacts with mouse and rat AKT2 protein.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
Immunohistochemistry (0.5-2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from HeLa was immunoblotted by AKT2 polyclonal antibody (Cat # PAB12651) at 1 : 500.Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT2
Entrez GeneID
208Gene Name
AKT2
Gene Alias
PKBB, PKBBETA, PRKBB, RAC-BETA
Gene Description
v-akt murine thymoma viral oncogene homolog 2
Gene Ontology
HyperlinkGene Summary
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Other Designations
Murine thymoma viral (v-akt) homolog-2|rac protein kinase beta
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Interactome
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Pathway
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Disease
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Publication Reference
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Phosphatidylinositol-3-OH Kinase (PI3K)/AKT2, activated in breast cancer, regulates and is induced by estrogen receptor alpha (ERalpha) via interaction between ERalpha and PI3K.
Sun M, Paciga JE, Feldman RI, Yuan Z, Coppola D, Lu YY, Shelley SA, Nicosia SV, Cheng JQ.
Cancer Research 2001 Aug; 61(16):5985.
Application:IP, WB, Human, Human breast tumor specimens.
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Phosphatidylinositol-3-OH Kinase (PI3K)/AKT2, activated in breast cancer, regulates and is induced by estrogen receptor alpha (ERalpha) via interaction between ERalpha and PI3K.
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