TFPI (Human) ELISA Kit
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Specifications
Product Description
TFPI (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human TFPI.
Suitable Sample
Cell Culture Supernatant, CSF, Milk, Plasma, Serum
Sample Volume
50 uL
Label
Peroxidase-conjugated
Detection Method
Colorimetric
Assay Type
Quantitative
Intra-Assay
4.6%
Inter-Assay
7.3%
Spiking Recovery
98.50%
Calibration Range
0.156 to 10 ng/mL
Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store components of the kit at 4°C or -20°C as described in the protocol.
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Applications
Quantification
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Gene Info — TFPI
Entrez GeneID
7035Gene Name
TFPI
Gene Alias
EPI, LACI, TFI, TFPI1
Gene Description
tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
Omim ID
152310Gene Ontology
HyperlinkGene Summary
This gene encodes a protease inhibitor that regulates the tissue factor (TF)-dependent pathway of blood coagulation. The coagulation process initiates with the formation of a factor VIIa-TF complex, which proteolytically activates additional proteases (factors IX and X) and ultimately leads to the formation of a fibrin clot. The product of this gene inhibits the activated factor X and VIIa-TF proteases in an autoregulatory loop. The encoded protein is glycosylated and predominantly found in the vascular endothelium and plasma in both free forms and complexed with plasma lipoproteins. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been confirmed. [provided by RefSeq
Other Designations
OTTHUMP00000163472|anti-convertin|extrinsic pathway inhibitor|lipoprotein-associated coagulation inhibitor|tissue factor pathway inhibitor
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Tissue factor pathway inhibitor and the revised theory of coagulation.
Broze GJ Jr.
Annual Review of medicine 1995 Jan; 46:103.
Application:Quant, Human, Human plasma.
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The initiation of the tissue factor dependent pathway of blood coagulation.
Rapaport SI.
Adv Exp Med Biol 1990 Jan; 281:97.
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Purification and characterization of the lipoprotein-associated coagulation inhibitor from human plasma.
Novotny WF, Girard TJ, Miletich JP, Broze GJ Jr.
The Journal of Biological Chemistry 1989 Nov; 264(31):18832.
Application:Quant, Human, Human plasma.
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Tissue factor pathway inhibitor and the revised theory of coagulation.
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