Western blot using ABCB1 polyclonal antibody (Cat # PAB9962) shows detection of ABCB1 in crude membrane extracts from HF insect cells over-expressing human ABCB1. The extract was loaded onto a gel in the amounts indicated followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1 : 600, followed by Peroxidase Conjugated Affinity Purified Anti-Rabbit IgG at 1 : 10,000. Personal Communication, Anna Calcagno, CCR-NCI, Bethesda, MD.
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is an ATP-dependent drug efflux pump for xenobiotic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. [provided by RefSeq
ATP-binding cassette, subfamily B, member 1,P glycoprotein 1,P-glycoprotein 1,colchicin sensitivity,doxorubicin resistance,multidrug resistance 1,multidrug resistance protein 1