AIF1 polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
AIF1 polyclonal antibody (Cat # PAB7034) (1 ug/mL) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AIF1 polyclonal antibody (Cat # PAB7034, 2.5 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of AIF1.
Immunogen
A synthetic peptide corresponding to human AIF1.
Sequence
C-YEEKAREKEKP
Host
Goat
Theoretical MW (kDa)
10.5, 16.7
Reactivity
Human
Specificity
This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western blot (1-3 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
AIF1 polyclonal antibody (Cat # PAB7034) (1 ug/mL) staining of human lymph node lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
AIF1 polyclonal antibody (Cat # PAB7034, 2.5 ug/mL) staining of paraffin embedded human placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — AIF1
Entrez GeneID
199Protein Accession#
NP_116573.1;NP_001614.3Gene Name
AIF1
Gene Alias
AIF-1, IBA1, IRT-1
Gene Description
allograft inflammatory factor 1
Omim ID
601833Gene Ontology
HyperlinkGene Summary
This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000029354|OTTHUMP00000029356|interferon gamma responsive transcript|ionized calcium-binding adapter molecule
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Interactome
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Disease
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Publication Reference
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Expression of allograft inflammatory factor-1 in T lymphocytes: a role in T-lymphocyte activation and proliferative arteriopathies.
Kelemen SE, Autieri MV.
The American Journal of Pathology 2005 Aug; 167(2):619.
Application：IHC, WB, WB-Tr, Human, Coronary artery, PBMCs, MOLT-4 cells.
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Expression of allograft inflammatory factor-1 in T lymphocytes: a role in T-lymphocyte activation and proliferative arteriopathies.
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