AKT3 polyclonal antibody
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of AKT3.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S472 of human AKT3.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western blot analysis of AKT3 (arrow) using rabbit AKT3 polyclonal antibody (Cat # PAB4553). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT3 gene (Lane 2) (Origene Technologies).Enzyme-linked Immunoabsorbent Assay
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Gene Info — AKT3
Entrez GeneID
10000Protein Accession#
NP_005456;Q9Y243Gene Name
AKT3
Gene Alias
DKFZp434N0250, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Gene Description
v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Omim ID
611223Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000037911|OTTHUMP00000037912|RAC-gamma serine/threonine protein kinase|protein kinase B gamma|serine threonine protein kinase, Akt-3|v-akt murine thymoma viral oncogene homolog 3
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Transforming growth factor-beta1 stimulated protein kinase B serine-473 and focal adhesion kinase tyrosine phosphorylation dependent on cell adhesion in human hepatocellular carcinoma SMMC-7721 cells.
Xu Z, Ma DZ, Wang LY, Su JM, Zha XL.
Biochemical and Biophysical Research Communications 2003 Dec; 312(2):388.
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Defective signaling through Akt-2 and -3 but not Akt-1 in insulin-resistant human skeletal muscle: potential role in insulin resistance.
Brozinick JT Jr, Roberts BR, Dohm GL.
Diabetes 2003 Apr; 52(4):935.
Application:KA, Human, Human skeletal muscle.
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Gene expression profiling in prostate cancer cells with Akt activation reveals Fra-1 as an Akt-inducible gene.
Tiwari G, Sakaue H, Pollack JR, Roth RA.
Molecular Cancer Research: MCR 2003 Apr; 1(6):475.
Application:IP, KA, Human, PC-3 cells.
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Transforming growth factor-beta1 stimulated protein kinase B serine-473 and focal adhesion kinase tyrosine phosphorylation dependent on cell adhesion in human hepatocellular carcinoma SMMC-7721 cells.
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