MOS (Human) Recombinant Protein
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Result of activity analysis
Result of activity analysis
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Specification
Product Description
Human MOS (NP_005363.1, 1 a.a. - 346 a.a.) full-length recombinant protein with GST tag expressed in baculovirus infected Sf21 cells.
Host
insect
Theoretical MW (kDa)
65
Form
Liquid
Preparation Method
Baculovirus infected insect cell (Sf21) expression system
Purification
Glutathione sepharose chromatography
Purity
77 % by SDS-PAGE/CBB staining
Activity
The activity was determined by ELISA. The enzyme was incubated with GST-fused substrate protein, and after stopping kinase reaction by EDTA, the reaction solution was transferred into glutathione-coated plate. Phosphorylation was detected by anti-phospho antibody and HRP-labeled anti-rabbit IgG(or HRP-labeled anti-mouse IgG). Substrate: MAP2K1 [inactive mutant]. ATP: 100 uM.
Quality Control Testing
Loading 1 ug protein in SDS-PAGE
Storage Buffer
In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — MOS
Entrez GeneID
4342Protein Accession#
NP_005363.1Gene Name
MOS
Gene Alias
MGC119962, MGC119963, MSV
Gene Description
v-mos Moloney murine sarcoma viral oncogene homolog
Omim ID
190060Gene Ontology
HyperlinkGene Summary
MOS is a serine/threonine kinase that activates the MAP kinase cascade through direct phosphorylation of the MAP kinase activator MEK (MAP2K1; MIM 176872) (Prasad et al., 2008 [PubMed 18246541]).[supplied by OMIM
Other Designations
Oncogene MOS, Moloney murine sarcoma virus
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Interactome
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Pathway
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Disease
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