STAT4(phospho S722) & STAT4 Protein Phosphorylation Antibody Pair
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Specifications
Product Description
This protein phosphorylation antibody pair set comes with two antibodies, one against the STAT4 protein, and the other against the specific S722 phosphorylated site of STAT4 for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Dual recognition immunofluorescence result.
Representative image of Proximity Ligation Assay of protein phosphorylation. HeLa cells were stained with dual recognition antibody pair set, rabbit polyclonal antibody 1:1200 and mouse monoclonal antibody 1:50. Each red dot represents one single phosphorylated protein. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. Phospho-STAT4 S722 rabbit polyclonal antibody (20 ul)
With 0.09% sodium azide.
2. STAT4 mouse monoclonal antibody (40 ug)
In 1x PBS, pH 7.2
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — STAT4
Entrez GeneID
6775Gene Name
STAT4
Gene Alias
SLEB11
Gene Description
signal transducer and activator of transcription 4
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. [provided by RefSeq
Other Designations
OTTHUMP00000163553
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