UBE2I polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with UBE2I polyclonal antibody (Cat # PAB30654).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with UBE2I polyclonal antibody (Cat # PAB30654) shows strong nuclear positivity in trophoblastic cells.
Immunofluorescence
Immunofluorescent staining of U-2 OS with UBE2I polyclonal antibody (Cat # PAB30654) (Green) shows positivity in cytoplasm.
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human UBE2I.
Immunogen
Recombinant protein corresponding to human UBE2I.
Sequence
IALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQ
Host
Rabbit
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with UBE2I polyclonal antibody (Cat # PAB30654).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with UBE2I polyclonal antibody (Cat # PAB30654) shows strong nuclear positivity in trophoblastic cells.Immunofluorescence
Immunofluorescent staining of U-2 OS with UBE2I polyclonal antibody (Cat # PAB30654) (Green) shows positivity in cytoplasm. -
Gene Info — UBE2I
Entrez GeneID
7329Protein Accession#
P63279Gene Name
UBE2I
Gene Alias
C358B7.1, P18, UBC9
Gene Description
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Omim ID
601661Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
SUMO-1-protein ligase|ubiquitin carrier protein|ubiquitin conjugating enzyme 9|ubiquitin-conjugating enzyme E2I|ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)|ubiquitin-conjugating enzyme UbcE2A|ubiquitin-like protein SUMO-1 conjugating enzym
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Interactome
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Pathway
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Disease
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