UBE2I polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
UBE2I polyclonal antibody (Cat # PAB6303) staining (0.1 ug/mL) of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
UBE2I polyclonal antibody (Cat # PAB6303) staining (0.3 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of UBE2I.
Immunogen
A synthetic peptide corresponding to N-terminus of human UBE2I.
Sequence
SGIALSRLAQERK-C
Host
Goat
Theoretical MW (kDa)
18
Reactivity
Human, Mouse, Rat
Specificity
This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.1-0.3 ug/mL)
Immunohistochemistry (1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
UBE2I polyclonal antibody (Cat # PAB6303) staining (0.1 ug/mL) of human kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
UBE2I polyclonal antibody (Cat # PAB6303) staining (0.3 ug/mL) of paraffin embedded human kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — UBE2I
Entrez GeneID
7329Protein Accession#
NP_003336.1;NP_919235.1;NP_919236.1;NP_919237.1Gene Name
UBE2I
Gene Alias
C358B7.1, P18, UBC9
Gene Description
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Omim ID
601661Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
SUMO-1-protein ligase|ubiquitin carrier protein|ubiquitin conjugating enzyme 9|ubiquitin-conjugating enzyme E2I|ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)|ubiquitin-conjugating enzyme UbcE2A|ubiquitin-like protein SUMO-1 conjugating enzym
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Interactome
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Pathway
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Disease
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Publication Reference
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Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.
Wang ZY, Qiu QQ, Seufert W, Taguchi T, Testa JR, Whitmore SA, Callen DF, Welsh D, Shenk T, Deuel TF.
The Journal of Biological Chemistry 1996 Oct; 271(40):24811.
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Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9.
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