UBE2I polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of UBE2I.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human UBE2I.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
Western Blot (1:1000)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry
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Gene Info — UBE2I
Entrez GeneID
7329Protein Accession#
NP_003336;P63279Gene Name
UBE2I
Gene Alias
C358B7.1, P18, UBC9
Gene Description
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Omim ID
601661Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
SUMO-1-protein ligase|ubiquitin carrier protein|ubiquitin conjugating enzyme 9|ubiquitin-conjugating enzyme E2I|ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)|ubiquitin-conjugating enzyme UbcE2A|ubiquitin-like protein SUMO-1 conjugating enzym
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Interactome
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Pathway
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Disease
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Publication Reference
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Interaction of the developmental regulator SALL1 with UBE2I and SUMO-1.
Netzer C, Bohlander SK, Rieger L, Muller S, Kohlhase J.
Biochemical and Biophysical Research Communications 2002 Aug; 296(4):870.
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Associations of UBE2I with RAD52, UBL1, p53, and RAD51 proteins in a yeast two-hybrid system.
Shen Z, Pardington-Purtymun PE, Comeaux JC, Moyzis RK, Chen DJ.
Genomics 1996 Oct; 37(2):183.
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Assignment of the gene for a ubiquitin-conjugating enzyme (UBE2I) to human chromosome band 16p13.3 by in situ hybridization.
Tachibana M, Iwata N, Watanabe A, Nobukuni Y, Ploplis B, Kajigaya S.
Cytogenetics and Cell Genetics 1996 Jan; 75(4):222.
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Interaction of the developmental regulator SALL1 with UBE2I and SUMO-1.
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