UBE2I monoclonal antibody, clone 1B10
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) against HeLa (1), HepG2 (2), and COS-7 (3) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) against recombinant human UBE2I protein.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissue (A) and bladder cancer tissue (B) using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of HepG2 cells using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry
Flow cytometric analysis of HepG2 cells using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) (green) and negative control (red).
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant UBE2I.
Immunogen
Recombinant protein corresponding to human UBE2I.
Host
Mouse
Theoretical MW (kDa)
18
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Immunofluorescence (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) against HeLa (1), HepG2 (2), and COS-7 (3) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) against recombinant human UBE2I protein.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human prostate tissue (A) and bladder cancer tissue (B) using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) with DAB staining.Immunofluorescence
Immunofluorescence analysis of HepG2 cells using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of HepG2 cells using UBE2I monoclonal antobody, clone 1B10 (Cat # MAB10344) (green) and negative control (red). -
Gene Info — UBE2I
Entrez GeneID
7329Gene Name
UBE2I
Gene Alias
C358B7.1, P18, UBC9
Gene Description
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Omim ID
601661Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
SUMO-1-protein ligase|ubiquitin carrier protein|ubiquitin conjugating enzyme 9|ubiquitin-conjugating enzyme E2I|ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)|ubiquitin-conjugating enzyme UbcE2A|ubiquitin-like protein SUMO-1 conjugating enzym
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Interactome
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Pathway
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Disease
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