UBE2I recombinant monoclonal antibody, clone R01-6I6
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More Files
- More Functions
Western Blot
Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with UBE2I recombinant monoclonal antibody, clone R01-6I6 (Cat # RAB01207).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human UBE2I.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against synthetic peptide corresponding to human UBE2I/UBC9.
Theoretical MW (kDa)
Calculated MW: 18 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunohistochemistry (Frozen sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibodyImmunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemistry staining of HeLa cells with UBE2I recombinant monoclonal antibody, clone R01-6I6 (Cat # RAB01207).Immunofluorescence
Immunoprecipitation
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Gene Info — UBE2I
Entrez GeneID
7329Protein Accession#
P63279Gene Name
UBE2I
Gene Alias
C358B7.1, P18, UBC9
Gene Description
ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Omim ID
601661Gene Ontology
HyperlinkGene Summary
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
SUMO-1-protein ligase|ubiquitin carrier protein|ubiquitin conjugating enzyme 9|ubiquitin-conjugating enzyme E2I|ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9)|ubiquitin-conjugating enzyme UbcE2A|ubiquitin-like protein SUMO-1 conjugating enzym
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Interactome
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Pathway
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Disease
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