NFE2L2 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of NFE2L2.
Immunogen
A synthetic peptide correspoding to amino acids 445-458 at internal region of human NFE2L2.
Sequence
CKHSSRLEAHLTRDE
Host
Goat
Theoretical MW (kDa)
67.8
Reactivity
Human
Specificity
This antibody is expected to recognize all reported isoforms (NP_006155.2; NP_001138884.1; NP_001138885.1).
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
NFE2L2 polyclonal antibody (Cat # PAB19757) (0.3 ug/mL) staining of human lung tissue lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFE2L2
Entrez GeneID
4780Protein Accession#
NP_006155.2;NP_001138884.1;NP_001138885.1Gene Name
NFE2L2
Gene Alias
NRF2
Gene Description
nuclear factor (erythroid-derived 2)-like 2
Omim ID
600492Gene Ontology
HyperlinkGene Summary
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM
Other Designations
NF-E2-related factor 2|OTTHUMP00000164988
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Interactome
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Disease
- Adenoma
- Alzheimer Disease
- Asthma
- Atherosclerosis
- Breast cancer
- Breast Neoplasms
- Calcinosis
- Carcinoma
- Cataract
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