NFE2L2 (phospho S40) recombinant monoclonal antibody, clone 2E6
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 2: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 3: HepG2 whole cell lysate (not treated), Lane 4: 293 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 5: 293 whole cell lysate (not treated) with NFE2L2 (phospho S40) recombinant monoclonal antibody, clone 2E6 (Cat # RAB04237).
Immunohistochemistry
Immunohistochemical staining of human breast cancer with FNFE2L2 (phospho S40) recombinant monoclonal antibody, clone 2E6 (Cat # RAB04237) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of Hela cells with NFE2L2 (phospho S40) recombinant monoclonal antibody, clone 2E6 (Cat # RAB04237) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NFE2L2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S40 of human NFE2L2.
Theoretical MW (kDa)
Calculated MW: 90 kD
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: HepG2 whole cell lysate (treated with Calyculin A 100 nM/60 mins), Lane 2: HepG2 whole cell lysate (treated with EGF 100 ng/mL/20 mins), Lane 3: HepG2 whole cell lysate (not treated), Lane 4: 293 whole cell lysate (treated with Calyculin A 100 nM/60 mins) and Lane 5: 293 whole cell lysate (not treated) with NFE2L2 (phospho S40) recombinant monoclonal antibody, clone 2E6 (Cat # RAB04237).Immunohistochemistry
Immunohistochemical staining of human breast cancer with FNFE2L2 (phospho S40) recombinant monoclonal antibody, clone 2E6 (Cat # RAB04237) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of Hela cells with NFE2L2 (phospho S40) recombinant monoclonal antibody, clone 2E6 (Cat # RAB04237) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
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Gene Info — NFE2L2
Entrez GeneID
4780Protein Accession#
Q16236Gene Name
NFE2L2
Gene Alias
NRF2
Gene Description
nuclear factor (erythroid-derived 2)-like 2
Omim ID
600492Gene Ontology
HyperlinkGene Summary
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM
Other Designations
NF-E2-related factor 2|OTTHUMP00000164988
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Interactome
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Disease
- Adenoma
- Alzheimer Disease
- Asthma
- Atherosclerosis
- Breast cancer
- Breast Neoplasms
- Calcinosis
- Carcinoma
- Cataract
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