NFE2L2 (Phospho S40) recombinant monoclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded rat brain using anti using NFE2L2 (Phospho S40) recombinant monoclonal antibody (Cat # RAB02635). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human NFE2L2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against KLH conjugated Synthesised phosphopeptide derived from human NFE2L2 around the phosphorylation site of Ser40.
Theoretical MW (kDa)
100
Reactivity
Human
Specificity
This antibody detects endogenous levels of Nrf2 protein only when phosphorylated at Ser40.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:100-1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.01M TBS, pH7.4 (1% BSA, 0.03% Proclin300 and 50% Glycerol)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraformaldehyde-fixed, paraffin embedded rat brain using anti using NFE2L2 (Phospho S40) recombinant monoclonal antibody (Cat # RAB02635). Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation at 1:200 overnight at 4°C. -
Gene Info — NFE2L2
Entrez GeneID
4780Protein Accession#
Q16236Gene Name
NFE2L2
Gene Alias
NRF2
Gene Description
nuclear factor (erythroid-derived 2)-like 2
Omim ID
600492Gene Ontology
HyperlinkGene Summary
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM
Other Designations
NF-E2-related factor 2|OTTHUMP00000164988
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Disease
- Adenoma
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