NFE2L2 (phospho S40) recombinant monoclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane1:HepG2 whole cell lysate Lane2:Raji whole cell lysate with NFE2L2 (phospho S40) recombinant monoclonal antibody (Cat # RAB02747) at 1:1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFE2L2 (phospho S40) recombinant monoclonal antibody (Cat # RAB02747). Counter stained with hematoxylin.
Immunocytochemistry
Immunocytochemical staining of HeLa cells using NFE2L2 (phospho S40) recombinant monoclonal antibody (Cat # RAB02747)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against NFE2L2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant NFE2L2.
Theoretical MW (kDa)
68, 90
Reactivity
Human
Specificity
This antibody detects endogenous levels of Nrf2 only when phosphorylated at Ser40.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50-1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:HepG2 whole cell lysate Lane2:Raji whole cell lysate with NFE2L2 (phospho S40) recombinant monoclonal antibody (Cat # RAB02747) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFE2L2 (phospho S40) recombinant monoclonal antibody (Cat # RAB02747). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of HeLa cells using NFE2L2 (phospho S40) recombinant monoclonal antibody (Cat # RAB02747)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
Flow Cytometry
-
Gene Info — NFE2L2
Entrez GeneID
4780Protein Accession#
Q16236Gene Name
NFE2L2
Gene Alias
NRF2
Gene Description
nuclear factor (erythroid-derived 2)-like 2
Omim ID
600492Gene Ontology
HyperlinkGene Summary
NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM
Other Designations
NF-E2-related factor 2|OTTHUMP00000164988
-
Interactome
-
Disease
- Adenoma
- Alzheimer Disease
- Asthma
- Atherosclerosis
- Breast cancer
- Breast Neoplasms
- Calcinosis
- Carcinoma
- Cataract
+ View More Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com