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Last updated: 2017/9/24
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PARK7 polyclonal antibody

  • Catalog # : PAB10070
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of PARK7.
  • Immunogen:
  • A synthetic peptide corresponding to amino acids 177-189 of human PARK7.
  • Host:
  • Rabbit
  • Reactivity:
  • Chicken, Dog, Frog, Human, Mouse, Rat
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Synthetic Peptide.
  • Recommend Usage:
  • ELISA (1:10000-1:50000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (2-5 ug/mL)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis is shown using PARK7 polyclonal antibody (Cat # PAB10070) to detect PARK7 present in Jurkat whole cell lysate.
    This western blot shows reactivity with human PARK7 protein.
    Comparison to a molecular weight marker indicates a predominant band of ~28.0 KDa.
    Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown).
    A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose.
    The blot was incubated with a 1:1,300 dilution ofthe antibody overnight at 4°C followed by detectionusing IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at RT.
    IRDye™800fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
    IRDyeis a trademark of LI-COR, Inc.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry of PARK7 polyclonal antibody (Cat # PAB10070) was used at a 5 ug/mL to detect PARK7 in a variety of tissues.
    In some tissues elevated background stainingwas noted.
    In these instances further optimization of dilution is suggested.
    This image shows PARK7 staining of human pancreas.
    Tissue was formalin-fixed and paraffin embedded.
    Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Protein Accession#:
  • Q99497;NP_009193
  • Gene Name:
  • PARK7
  • Gene Alias:
  • DJ-1,DJ1,FLJ27376,FLJ34360,FLJ92274
  • Gene Description:
  • Parkinson disease (autosomal recessive, early onset) 7
  • Gene Summary:
  • The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000001348,OTTHUMP00000001349,OTTHUMP00000001350,OTTHUMP00000001351,Parkinson disease protein 7,oncogene DJ1,protein DJ-1
  • Gene Pathway
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