Western blot analysis is shown using PARK7 polyclonal antibody (Cat # PAB10070) to detect PARK7 present in Jurkat whole cell lysate. This western blot shows reactivity with human PARK7 protein. Comparison to a molecular weight marker indicates a predominant band of ~28.0 KDa. Peptide competition blocks specific reactivity of the antibody with PARK7 (not shown). A 16% Tris-Tricine gel was used to separate proteins prior to transfer to 0.2 um nitrocellulose. The blot was incubated with a 1:1,300 dilution ofthe antibody overnight at 4°C followed by detectionusing IRDye™800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min at RT. IRDye™800fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDyeis a trademark of LI-COR, Inc.
Immunohistochemistry of PARK7 polyclonal antibody (Cat # PAB10070) was used at a 5 ug/mL to detect PARK7 in a variety of tissues. In some tissues elevated background stainingwas noted. In these instances further optimization of dilution is suggested. This image shows PARK7 staining of human pancreas. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush,Life Span Biosciences, Seattle, WA.
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
OTTHUMP00000001348,OTTHUMP00000001349,OTTHUMP00000001350,OTTHUMP00000001351,Parkinson disease protein 7,oncogene DJ1,protein DJ-1