PARK7 recombinant monoclonal antibody, clone R04-6H5
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human PARK7/DJ1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human PARK7/DJ1
Theoretical MW (kDa)
Calculated MW: 20 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of PARK7/DJ1 in K562, rat Brain, C6, 3T3, Hela lysates using human PARK7/DJ1 recombinant monoclonal antibody, clone R04-6H5 (Cat # RAB01692).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of Human tonsil with PARK7/DJ1 recombinant monoclonal antibody, clone R04-6H5 (Cat # RAB01692).Immunohistochemistry (Frozen sections)
Immunocytochemistry
Immunocytochemical staining of Hela with PARK7/DJ1 recombinant monoclonal antibody, clone R04-6H5 (Cat # RAB01692). (A) PARK7/DJ1 (green) and (B) DAPI (blue).Immunoprecipitation
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Gene Info — PARK7
Entrez GeneID
11315Protein Accession#
Q99497Gene Name
PARK7
Gene Alias
DJ-1, DJ1, FLJ27376, FLJ34360, FLJ92274
Gene Description
Parkinson disease (autosomal recessive, early onset) 7
Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000001348|OTTHUMP00000001349|OTTHUMP00000001350|OTTHUMP00000001351|Parkinson disease protein 7|oncogene DJ1|protein DJ-1
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Interactome
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Disease
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