Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with RB1 (phospho S788) polyclonal antibody (Cat # PAB0599) which was peroxidase-conjugated to the secondary antibody followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Dot Blot (Peptide)
Dot blot analysis of RB1 (phospho S788) polyclonal antibody (Cat # PAB0599) on nitrocellulose membrane. 50 ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots.
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
OTTHUMP00000018397,retinoblastoma suspectibility protein,retinoblastoma-associated protein