RB1 (phospho S780) recombinant monoclonal antibody, clone 2E9
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More Files
- More Functions
Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with RB1 (phospho S780) recombinant monoclonal antibody, clone 2E9 (Cat # RAB04300) (diluated at 1:100).
Immunofluorescence
Immunofluorescent staining of K562 cells with RB1 (phospho S780) recombinant monoclonal antibody, clone 2E9 (Cat # RAB04300) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with RB1 (phospho S780) recombinant monoclonal antibody, clone 2E9 (Cat # RAB04300).
Lane 1: rabbit control IgG, Lane 2: RAB04300 precipitates and Lane 3: Input (Hela whole cell lysates).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human RB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic phosphopeptide corresponding to residues surrounding S780 of human RB1.
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:200-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry
Immunohistochemical staining of human breast carcinoma with RB1 (phospho S780) recombinant monoclonal antibody, clone 2E9 (Cat # RAB04300) (diluated at 1:100).Immunofluorescence
Immunofluorescent staining of K562 cells with RB1 (phospho S780) recombinant monoclonal antibody, clone 2E9 (Cat # RAB04300) (diluated at 1:100). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Immunoprecipitation
Immunoprecipitation analysis of HeLa cell lysate with RB1 (phospho S780) recombinant monoclonal antibody, clone 2E9 (Cat # RAB04300).
Lane 1: rabbit control IgG, Lane 2: RAB04300 precipitates and Lane 3: Input (Hela whole cell lysates).Enzyme-linked Immunoabsorbent Assay
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Gene Info — RB1
Entrez GeneID
5925Protein Accession#
P06400Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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