RB1 (phospho S807) recombinant monoclonal antibody
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
-
Specification
Product Description
Rabbit recombinant monoclonal antibody raised against RB1.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant RB1.
Theoretical MW (kDa)
106
Reactivity
Human, Mouse, Rat
Specificity
This antibody detects endogenous levels of Rb protein only when phosphorylated at Ser807.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.2 (50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at 4°C short term.
Aliquot and store at -20°C long term.
Avoid freeze-thaw cycles.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot analysis of Lane1:A549 whole cell lysate with RB1 (phospho S807) recombinant monoclonal antibody (Cat # RAB02705) at 1:1000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using RB1 (phospho S807) recombinant monoclonal antibody (Cat # RAB02705). Counter stained with hematoxylin.Immunocytochemistry
Immunocytochemical staining of A549 cells using RB1 (phospho S807) recombinant monoclonal antibody (Cat # RAB02705)(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton *100/PBS.Immunofluorescence
-
Gene Info — RB1
Entrez GeneID
5925Protein Accession#
P06400Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
-
Interactome
-
Pathway
-
Disease
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com