Western blot analysis of cell lysates of 293T (30 ug) were resolved by SDS - PAGE , transferred to NC membrane and probed with NAMPT monoclonal antibody , clone 4D5 (1 : 1000) (Cat # MAB1122). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. This gene has a pseudogene on chromosome 10. [provided by RefSeq