HDAC2 recombinant monoclonal antibody, clone 9H4
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of MCF-7 whole cell lysate, K562 whole cell lysate with HDAC2 recombinant monoclonal antibody, clone 9H4 (Cat # RAB07405).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer using HDAC2 recombinant monoclonal antibody, clone 9H4 (Cat # RAB07405) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Immunofluorescence
Immunofluorescent staining of Hela Cells with HDAC2 recombinant monoclonal antibody, clone 9H4 (Cat # RAB07405). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HDAC2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HDAC2.
Theoretical MW (kDa)
Calculated MW: 56, 5
Reactivity
Human
Form
Liquid
Purification
Affinity chromatography purification
Isotype
IgG
Recommend Usage
ELISA
Immunofluorescence (1:20-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150 mM NaCl, 0.02% sodium azide and 50% glycerol)
Storage Instruction
Store at -20°C or -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of MCF-7 whole cell lysate, K562 whole cell lysate with HDAC2 recombinant monoclonal antibody, clone 9H4 (Cat # RAB07405).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human breast cancer using HDAC2 recombinant monoclonal antibody, clone 9H4 (Cat # RAB07405) on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.Immunofluorescence
Immunofluorescent staining of Hela Cells with HDAC2 recombinant monoclonal antibody, clone 9H4 (Cat # RAB07405). The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDAC2
Entrez GeneID
3066Protein Accession#
Q92769Gene Name
HDAC2
Gene Alias
RPD3, YAF1
Gene Description
histone deacetylase 2
Omim ID
605164Gene Ontology
HyperlinkGene Summary
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq
Other Designations
OTTHUMP00000040427|YY1-associated factor 1|transcriptional regulator homolog RPD3
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Interactome
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Pathway
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Disease
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