HDAC2 monoclonal antibody, clone AAE-8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of K562 cell lysate with HDAC2 monoclonal antibody, clone AAE-8 (Cat # MAB20123).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast with HDAC2 monoclonal antibody, clone AAE-8 (Cat # MAB20123).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human HDAC2.
Immunogen
A synthetic peptide corresponding to human HDAC2.
Host
Rabbit
Theoretical MW (kDa)
55.364
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:50)
Immunocytochemistry (1:50-1:100)
Immunofluorescence (1:50-1:100)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100)
Immunoprecipitation (1:30)
Western Blot (1:1000-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of K562 cell lysate with HDAC2 monoclonal antibody, clone AAE-8 (Cat # MAB20123).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast with HDAC2 monoclonal antibody, clone AAE-8 (Cat # MAB20123).Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — HDAC2
Entrez GeneID
3066Protein Accession#
Q92769Gene Name
HDAC2
Gene Alias
RPD3, YAF1
Gene Description
histone deacetylase 2
Omim ID
605164Gene Ontology
HyperlinkGene Summary
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq
Other Designations
OTTHUMP00000040427|YY1-associated factor 1|transcriptional regulator homolog RPD3
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Interactome
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Pathway
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Disease
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