HDAC2 recombinant monoclonal antibody, clone 9E9
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HDAC2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human HDAC2.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: Hela whole cell lysate; Lane 2: HepG2 whole cell lysate; Lane 3: Jurkat whole cell lysate; Lane 4: MCF-7 whole cell lysate; Lane 5: K562 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of HDAC2 recombinant monoclonal antibody, clone 9E9 diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — HDAC2
Entrez GeneID
3066Protein Accession#
Q92769Gene Name
HDAC2
Gene Alias
RPD3, YAF1
Gene Description
histone deacetylase 2
Omim ID
605164Gene Ontology
HyperlinkGene Summary
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq
Other Designations
OTTHUMP00000040427|YY1-associated factor 1|transcriptional regulator homolog RPD3
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Interactome
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