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Western blot analysis of Lane 1: A431, Lane 2: Caco-2, Lane 3: MCF-7 and Lane 4: HepG2 with EPCAM recombinant monoclonal antibody, clone KS1/4 (Cat # RAB03432).
Western Blot analysis the lysates (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of with RAB03432 at 2 ug/mL before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescent staining of Caco-2 cells with EPCAM recombinant monoclonal antibody, clone KS1/4 (Cat # RAB03432).
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric r version of RAB03432 at a dilution of 1:100 for 1h followed by Alexa Fluor® 488 secondary antibody at a dilution of 1:1000, showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by Alexa Fluor® 488 secondary antibody.
(A) RAB03432
(B) DAPI
(C) Merged channels
(D) Isotype control

Flow cytometric analysis of Caco-2 cells with EPCAM recombinant monoclonal antibody, clone KS1/4 (Cat # RAB03432).
Paraformaldehyde fixed Caco-2 cells were stained with anti-unknown specificity antibody (isotype control-black line) or the r version of RAB03432 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.