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Western blot analysis of Lane 1: human breast carcinoma and Lane 2: human colorectal carcinoma tissue lysate with EPCAM recombinant monoclonal antibody, clone HEA125 (Cat # RAB03429).
Western Blot analysis the tissue lysate (35 ug protein in RIPA buffer) was resolved on an SDS PAGE gel and blots probed with the chimeric mouse IgG version of RAB03429 at 1 ug/mL before detection using an anti-mouse secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Immunofluorescent staining of Caco-2 cells with EPCAM recombinant monoclonal antibody, clone HEA125 (Cat # RAB03429).
Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells stained with the chimeric mouse IgG version of RAB03429 at 10 ug/mL followed by Alexa Fluor® 488 secondary antibody (2 ug/mL), showing membrane staining. The nuclear stain is DAPI (blue). The isotype control was stained with anti-unknown antibody followed by staining with Alexa Fluor® 488 secondary antibody.
(A) RAB03429
(B) DAPI
(C) Merged channels
(D) Isotype control

Flow cytometric analysis of Caco-2 cells with EPCAM recombinant monoclonal antibody, clone HEA125 (Cat # RAB03429).
Caco-2 cells were stained with anti-unknown IgG antibody (isotype control-black line) or the mouse IgG chimeric version of RAB03429 (blue line) at a dilution of 1:100 for 1h at RT. After washing the bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.