EPCAM recombinant monoclonal antibody, clone 6H12
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate.
Immunohistochemistry
Immunohistochemistry image of EPCAM recombinant monoclonal antibody, clone 6H12 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human EPCAM.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to full length human EPCAM.
Reactivity
Human
Form
Liquid
Purification
Affinity-chromatography
Isotype
IgG
Recommend Usage
ELISA
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: MCF-7 whole cell lysate.Immunohistochemistry
Immunohistochemistry image of EPCAM recombinant monoclonal antibody, clone 6H12 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EPCAM
Entrez GeneID
4072Protein Accession#
P16422Gene Name
EPCAM
Gene Alias
17-1A, 323/A3, CD326, CO-17A, CO17-1A, EGP, EGP-2, EGP34, EGP40, ESA, Ep-CAM, GA733-2, HEA125, KS1/4, KSA, M4S1, MH99, MIC18, MK-1, MOC31, TACST-1, TACSTD1, TROP1, hEGP-2
Gene Description
epithelial cell adhesion molecule
Omim ID
185535Gene Ontology
HyperlinkGene Summary
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq
Other Designations
adenocarcinoma-associated antigen|carcinoma-associated antigen GA733-2|human epithelial glycoprotein-2|membrane component, chromosome 4, surface marker (35kD glycoprotein)|tumor-associated calcium signal transducer 1
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Interactome
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Disease
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