HTRA2 recombinant monoclonal antibody, clone R06-9E4
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More Files
- More Functions
Western Blot
Western Blot analysis of Lane 1: 3T3 and Lane 2: Hela lysates with HTRA2 recombinant monoclonal antibody, clone R06-9E4 (Cat # RAB02192).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human HTRA2.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human HTRA2.
Theoretical MW (kDa)
Calculated MW: 49 kD
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence(1:50-1:200)
Immunohistochemistry (1:50-1:100)
Immunoprecipitation(1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at -20 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of Lane 1: 3T3 and Lane 2: Hela lysates with HTRA2 recombinant monoclonal antibody, clone R06-9E4 (Cat # RAB02192).Immunohistochemistry
Immunofluorescence
Immunoprecipitation
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Gene Info — HTRA2
Entrez GeneID
27429Protein Accession#
O43464Gene Name
HTRA2
Gene Alias
OMI, PARK13, PRSS25
Gene Description
HtrA serine peptidase 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
Other Designations
HtrA-like serine protease|Omi stress-regulated endoprotease|high temperature requirement protein A2|protease, serine, 25
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Interactome
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Disease
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