HTRA2 monoclonal antibody, clone EEH-8
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with HTRA2 monoclonal antibody, clone EEH-8 (Cat # MAB20353).
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human HTRA2.
Immunogen
A synthetic peptide corresponding to human HTRA2.
Host
Rabbit
Theoretical MW (kDa)
48.841
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:30)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Jurkat cell lysate with HTRA2 monoclonal antibody, clone EEH-8 (Cat # MAB20353).Immunohistochemistry
Immunoprecipitation
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Gene Info — HTRA2
Entrez GeneID
27429Protein Accession#
O43464Gene Name
HTRA2
Gene Alias
OMI, PARK13, PRSS25
Gene Description
HtrA serine peptidase 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
Other Designations
HtrA-like serine protease|Omi stress-regulated endoprotease|high temperature requirement protein A2|protease, serine, 25
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Interactome
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Disease
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