HTRA2 monoclonal antibody, clone 6H7A8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: HTRA2-hIgGFc transfected HEK293 cell lysates with HTRA2 monoclonal antibody, clone 6H7A8 (Cat # MAB21500).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with HTRA2 monoclonal antibody, clone 6H7A8 (Cat # MAB21500).
Flow Cytometry
Flow cytometric analysis of HeLa cells with HTRA2 monoclonal antibody, clone 6H7A8 (Cat # MAB21500) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human HTRA2.
Immunogen
Recombinant protein corresponding to amino acids 278-458 of human HTRA2.
Host
Mouse
Theoretical MW (kDa)
48.8
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: HTRA2-hIgGFc transfected HEK293 cell lysates with HTRA2 monoclonal antibody, clone 6H7A8 (Cat # MAB21500).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with HTRA2 monoclonal antibody, clone 6H7A8 (Cat # MAB21500).Flow Cytometry
Flow cytometric analysis of HeLa cells with HTRA2 monoclonal antibody, clone 6H7A8 (Cat # MAB21500) (Green). Red: Negative Control. -
Gene Info — HTRA2
Entrez GeneID
27429Protein Accession#
O43464Gene Name
HTRA2
Gene Alias
OMI, PARK13, PRSS25
Gene Description
HtrA serine peptidase 2
Gene Ontology
HyperlinkGene Summary
This gene encodes a serine protease. The protein has been localized in the endoplasmic reticulum and interacts with an alternatively spliced form of mitogen-activated protein kinase 14. The protein has also been localized to the mitochondria with release to the cytosol following apoptotic stimulus. The protein is thought to induce apoptosis by binding the apoptosis inhibitory protein baculoviral IAP repeat-containing 4. Nuclear localization of this protein has also been observed. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq
Other Designations
HtrA-like serine protease|Omi stress-regulated endoprotease|high temperature requirement protein A2|protease, serine, 25
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Interactome
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Disease
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