STK4 recombinant monoclonal antibody, clone R09-3C1
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More Files
- More Functions
Western Blot
Western blot analysis of MSP/MST1 in K562, rat Brain, C6, 3T3, Hela lysates using human MSP/MST1 recombinant monoclonal antibody, clone R09-3C1 (Cat # RAB01730).
Immunocytochemistry
Immunocytochemistry analysis of MSP (green) in K562 using human MSP recombinant monoclonal antibody, clone R09-3C1 (Cat # RAB01730), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human Serine/threonine-protein kinase 4.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human Serine/threonine-protein kinase 4
Theoretical MW (kDa)
Calculated MW: 56 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of MSP/MST1 in K562, rat Brain, C6, 3T3, Hela lysates using human MSP/MST1 recombinant monoclonal antibody, clone R09-3C1 (Cat # RAB01730).Immunocytochemistry
Immunocytochemistry analysis of MSP (green) in K562 using human MSP recombinant monoclonal antibody, clone R09-3C1 (Cat # RAB01730), and DAPI(blue).Immunoprecipitation
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Gene Info — STK4
Entrez GeneID
6789Protein Accession#
Q13043Gene Name
STK4
Gene Alias
DKFZp686A2068, KRS2, MST1, YSK3
Gene Description
serine/threonine kinase 4
Omim ID
604965Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq
Other Designations
OTTHUMP00000043418|dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2))|kinase responsive to stress 2|mammalian sterile 20-like 1|yeast Ste20-like
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Interactome
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Pathway
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Disease
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