STK4/STK3 (phospho T183) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with STK4/STK3 (phospho T183) polyclonal antibody (Cat # PAB29650) at 1:500-1:3000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with STK4/STK3 (phospho T183) polyclonal antibody (Cat # PAB29650) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human STK4/STK3.
Immunogen
Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T183 of human STK4/STK3.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse
Specificity
STK4/STK3 (phospho T183) polyclonal antibody detects endogenous levels of human STK4/STK3 only when phosphorylated at threonine 183.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunohistochemistry (1:50-1:100)
Western Blot (1:500-1:3000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cell lysates, Lane 2: Synthesized peptide treated 293 cell lysates reacted with STK4/STK3 (phospho T183) polyclonal antibody (Cat # PAB29650) at 1:500-1:3000 dilution.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of paraffin-embedded human brain tissue reacted with STK4/STK3 (phospho T183) polyclonal antibody (Cat # PAB29650) at 1:50-1:100 dilution. The picture on the right is treated with the synthesized peptide. -
Gene Info — STK3
Entrez GeneID
6788Protein Accession#
Q13043;Q13188Gene Name
STK3
Gene Alias
FLJ90748, KRS1, MST2
Gene Description
serine/threonine kinase 3 (STE20 homolog, yeast)
Omim ID
605030Gene Ontology
HyperlinkGene Summary
Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM
Other Designations
serine/threonine kinase 3 (Ste20, yeast homolog)
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Gene Info — STK4
Entrez GeneID
6789Protein Accession#
Q13043;Q13188Gene Name
STK4
Gene Alias
DKFZp686A2068, KRS2, MST1, YSK3
Gene Description
serine/threonine kinase 4
Omim ID
604965Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq
Other Designations
OTTHUMP00000043418|dJ211D12.2 (serine/threonine kinase 4 (MST1, KRS2))|kinase responsive to stress 2|mammalian sterile 20-like 1|yeast Ste20-like
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Interactome
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