HDAC2 (phospho S394) polyclonal antibody
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More Files
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Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells treated with UV (20 min) , using HDAC2 (phospho S394) polyclonal antibody (Cat # PAB7740).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Imunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HDAC2 (phospho S394) polyclonal antibody (Cat # PAB7740).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of HDAC2.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding S394 of human HDAC2.
Host
Rabbit
Theoretical MW (kDa)
60
Reactivity
Human, Mouse, Rat
Form
Liquid
Purification
Immunoaffinity purification
Concentration
1 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of extracts from HT-29 cells treated with UV (20 min) , using HDAC2 (phospho S394) polyclonal antibody (Cat # PAB7740).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Imunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HDAC2 (phospho S394) polyclonal antibody (Cat # PAB7740).Immunofluorescence
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Gene Info — HDAC2
Entrez GeneID
3066Protein Accession#
Q92769Gene Name
HDAC2
Gene Alias
RPD3, YAF1
Gene Description
histone deacetylase 2
Omim ID
605164Gene Ontology
HyperlinkGene Summary
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues on the N-terminal region of the core histones (H2A, H2B, H3 and H4). This protein also forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation, cell cycle progression and developmental events. [provided by RefSeq
Other Designations
OTTHUMP00000040427|YY1-associated factor 1|transcriptional regulator homolog RPD3
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Interactome
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Pathway
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Disease
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