MAD2L1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
MAD2L1 polyclonal antibody (Cat # PAB6237, 2 ug/mL) staining of HEK293 lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of MAD2L1.
Immunogen
A synthetic peptide corresponding to human MAD2L1.
Sequence
C-KVNSMVAYKIPVND
Host
Goat
Theoretical MW (kDa)
23.5
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
MAD2L1 polyclonal antibody (Cat # PAB6237, 2 ug/mL) staining of HEK293 lysate (35 ug protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAD2L1
Entrez GeneID
4085Protein Accession#
NP_002349Gene Name
MAD2L1
Gene Alias
HSMAD2, MAD2
Gene Description
MAD2 mitotic arrest deficient-like 1 (yeast)
Omim ID
601467Gene Ontology
HyperlinkGene Summary
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq
Other Designations
MAD2 (mitotic arrest deficient, yeast, homolog)-like 1|MAD2-like 1|MAD2-like protein 1|mitotic arrest deficient, yeast, homolog-like 1
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Interactome
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Pathway
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Disease
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Publication Reference
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Identification of a human mitotic checkpoint gene: hsMAD2.
Y Li, R Benezra.
Science 1996 Oct; 274(5285):246.
Application：Func, IF, WB-Ce, WB-Tr, Human, BT474, HeLa, MCF-7, MDA-MB-231, MDA-MB-453, MDA-MB-468, RH1, T-47D, cells.
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Identification of a human mitotic checkpoint gene: hsMAD2.
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