MAD2L1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot using MAD2L1 polyclonal antibody (Cat # PAB10076) shows detection of a predominant band at ~24 KDa corresponding to MAD2L1 (arrowhead) present in Jurkat (Lane 1) and HeLa (Lane 2) whole cell lysates using the 800 nmchannel (green).
The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins.
Specific band reactivity is blocked when the antibody is pre-incubated with immunizingpeptide (lanes 4 and 5 respectively) which completely blocks antibody staining.
Approximately 35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1200.
Incubation was 2h at room temperature followed by washes andreaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
Molecular weight markers were used for size comparison using the 700 nm channel (Lane 3). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of MAD2L1.
Immunogen
A synthetic peptide corresponding to amino acids 3-13 of human MAD2L1.
Host
Rabbit
Reactivity
Human
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:2000-1:10000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot using MAD2L1 polyclonal antibody (Cat # PAB10076) shows detection of a predominant band at ~24 KDa corresponding to MAD2L1 (arrowhead) present in Jurkat (Lane 1) and HeLa (Lane 2) whole cell lysates using the 800 nmchannel (green).
The identity of the higher molecular weight bands is unknown, although they may represent complexes of MAD2L1 with related binding proteins.
Specific band reactivity is blocked when the antibody is pre-incubated with immunizingpeptide (lanes 4 and 5 respectively) which completely blocks antibody staining.
Approximately 35 ug of lysate was separated on a 4-20% Tris-glycine gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1200.
Incubation was 2h at room temperature followed by washes andreaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MXHu for 45 min at room temperature.
Molecular weight markers were used for size comparison using the 700 nm channel (Lane 3). IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
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Gene Info — MAD2L1
Entrez GeneID
4085Protein Accession#
Q13257;NP_002349Gene Name
MAD2L1
Gene Alias
HSMAD2, MAD2
Gene Description
MAD2 mitotic arrest deficient-like 1 (yeast)
Omim ID
601467Gene Ontology
HyperlinkGene Summary
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq
Other Designations
MAD2 (mitotic arrest deficient, yeast, homolog)-like 1|MAD2-like 1|MAD2-like protein 1|mitotic arrest deficient, yeast, homolog-like 1
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Interactome
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Pathway
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Disease
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Publication Reference
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A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint.
Wang RH, Yu H, Deng CX.
PNAS 2004 Dec; 101(49):17108.
Application：WB-Tr, Human, Mouse , MEFs, UBR60 cells.
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Elevated level of spindle checkprotein MAD2 correlates with cellular mitotic arrest, but not with aneuploidy and clinicopathological characteristics in gastric cancer.
Wu CW, Chi CW, Huang TS.
World Journal of Gastroenterology 2004 Nov; 10(22):3240.
Application：WB-Ti, Human, Human gastric cancer.
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Mad2 and p27 expression profiles in colorectal cancer and its clinical significance.
Li GQ, Zhang HF.
World Journal of Gastroenterology 2004 Nov; 10(21):3218.
Application：IHC-P, Human, Human tubular adenocarcinomas.
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A requirement for breast-cancer-associated gene 1 (BRCA1) in the spindle checkpoint.
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