MAD2L1 monoclonal antibody, clone 17D10
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Western Blot (Cell lysate)
Western blot using MAD2L1 monoclonal antibody, clone 17D10 (Cat # MAB1926) shows detection of aband at ~24 KDa (arrowhead) correspondingto MAD2L1 present in a HeLa whole cell lysate (Lane 1).
Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE.
After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200.
The membrane was washed and reacted with a 1 : 5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 minat room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® InfraredImaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Otherdetection systems will yield similar results.
-
Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant.
Immunogen
Recombinant protein corresponding to full length human MAD2L1.
Host
Mouse
Reactivity
Human
Form
Liquid
Isotype
IgG1
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Recommend Usage
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Applications
Western Blot (Cell lysate)
Western blot using MAD2L1 monoclonal antibody, clone 17D10 (Cat # MAB1926) shows detection of aband at ~24 KDa (arrowhead) correspondingto MAD2L1 present in a HeLa whole cell lysate (Lane 1).
Approximately 75 ug of lysate was separated by 4-20% TG SDS-PAGE.
After blocking, the membrane was probed overnight at 4°C with the primary antibody diluted to 1:200.
The membrane was washed and reacted with a 1 : 5,000 dilution of IRDye™800 conjugated Sh-a-Mouse IgG [H&L] for 45 minat room temperature (800 nm channel, green).
Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
IRDye™800 fluorescence image was captured using the Odyssey® InfraredImaging System developed by LI-COR.
IRDye is a trademark of LI-COR, Inc. Otherdetection systems will yield similar results.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
-
Gene Info — MAD2L1
Entrez GeneID
4085Protein Accession#
Q13257;NP_002349Gene Name
MAD2L1
Gene Alias
HSMAD2, MAD2
Gene Description
MAD2 mitotic arrest deficient-like 1 (yeast)
Omim ID
601467Gene Ontology
HyperlinkGene Summary
MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq
Other Designations
MAD2 (mitotic arrest deficient, yeast, homolog)-like 1|MAD2-like 1|MAD2-like protein 1|mitotic arrest deficient, yeast, homolog-like 1
-
Interactome
-
Pathway
-
Disease
-
Publication Reference
-
Kinetochore structure and function.
Chan GK, Liu ST, Yen TJ.
Trends in Cell Biology 2005 Nov; 15(11):589.
Application：IF, WB, Human, Human mammalian cells.
-
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo.
Joseph J, Liu ST, Jablonski SA, Yen TJ, Dasso M.
Current Biology 2004 Apr; 14(7):611.
Application：IF, Human, RGG cells.
-
Purification of the mitotic checkpoint complex, an inhibitor of the APC/C from HeLa cells.
Sudakin V, Yen TJ.
Methods in Molecular Biology 2004 Jan; 281:199.
Application：WB, Human, HeLa cells.
-
Kinetochore structure and function.
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com