SRC polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
Western blot analysis of SRC polyclonal antibody (Cat # PAB3435) in mouse kidney tissue lysate. SRC (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SRC polyclonal antibody (Cat # PAB3435) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of SRC.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SRC.
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Recommend Usage
Immunofluorescence (1:10-50)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-100)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Western blot analysis of SRC polyclonal antibody (Cat # PAB3435) in mouse kidney tissue lysate. SRC (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human cancer tissue reacted with SRC polyclonal antibody (Cat # PAB3435) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.Immunofluorescence
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Gene Info — SRC
Entrez GeneID
6714Protein Accession#
P12931Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Interactome
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Pathway
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Disease
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Publication Reference
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Coexpression of IGF-1R and c-Src proteins in human pancreatic ductal adenocarcinoma.
Hakam A, Fang Q, Karl R, Coppola D.
Digestive Diseases and Sciences 2003 Oct; 48(10):1972.
Application：IHC-P, Human, Pancreatic ductal adenocarcinomas.
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An increase in the expression and total activity of endogenous p60(c-Src) in several factor-independent mutants of a human GM-CSF-dependent leukemia cell line (TF-1).
Horn S, Meyer J, Stocking C, Ostertag W, Jucker M.
Oncogene 2003 Oct; 22(46):7170.
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v-SRC specifically regulates the nucleo-cytoplasmic delocalization of the major isoform of TEL (ETV6).
Lopez RG, Carron C, Ghysdael J.
The Journal of Biological Chemistry 2003 Oct; 278(42):41316.
Application：IF, Human, HeLa cells.
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Coexpression of IGF-1R and c-Src proteins in human pancreatic ductal adenocarcinoma.
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