SRC recombinant monoclonal antibody, clone 21H5
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: NIH/3T3 whole cell lysate and Lane 2: MCF-7 whole cell lysate with SRC recombinant monoclonal antibody, clone 21H5 (Cat # RAB04355).
Immunofluorescence
Immunofluorescent staining of MCF-7 cells with SRC recombinant monoclonal antibody, clone 21H5 (Cat # RAB04355) (diluated at 1:39). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).
Flow Cytometry
Flow cytometric analysis of SH-SY5Y cells with SRC recombinant monoclonal antibody, clone 21H5 (Cat # RAB04355) (diluated at 1:50; purple line) and negative control (green line).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human SRC.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human SRC.
Theoretical MW (kDa)
Calculated MW: 60, 6
Reactivity
Human, Mouse
Form
Liquid
Purification
Affinity chromatography
Isotype
IgG
Recommend Usage
ELISA
Flow Cytometry
Immunofluorescence (1:20-1:200)
Western Blot (1:500-1:5000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH7.4 (150mM NaCl, 50% glycerol and 0.02% sodium azide)
Storage Instruction
Store at -20 °C or -80 °C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: NIH/3T3 whole cell lysate and Lane 2: MCF-7 whole cell lysate with SRC recombinant monoclonal antibody, clone 21H5 (Cat # RAB04355).Immunofluorescence
Immunofluorescent staining of MCF-7 cells with SRC recombinant monoclonal antibody, clone 21H5 (Cat # RAB04355) (diluated at 1:39). The secondary antibody was Alexa Fluor 488-congugated goat anti-rabbit IgG (green). Counter-stain DAPI was used (blue).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometric analysis of SH-SY5Y cells with SRC recombinant monoclonal antibody, clone 21H5 (Cat # RAB04355) (diluated at 1:50; purple line) and negative control (green line). -
Gene Info — SRC
Entrez GeneID
6714Protein Accession#
P12931Gene Name
SRC
Gene Alias
ASV, SRC1, c-SRC, p60-Src
Gene Description
v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Omim ID
190090Gene Ontology
HyperlinkGene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000174476|OTTHUMP00000174477|proto-oncogene tyrosine-protein kinase SRC|protooncogene SRC, Rous sarcoma|tyrosine kinase pp60c-src|tyrosine-protein kinase SRC-1
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Interactome
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Pathway
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Disease
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