RB1 (phospho S811) polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cells, Lane 2: Insulin treated 293 cells, Lane 3: Untreated HeLa cells, Lane 4: UV treated HeLa cells with RB1 (phospho S811) polyclonal antibody (Cat # PAB29596) at 1:500-1:2000 dilution.
Immunofluorescence
Immunofluorescent staining of U-2 OS cells with RB1 (phospho S811) polyclonal antibody (Cat # PAB29596) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining.
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic phosphopeptide of human RB1.
Immunogen
A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding S811 of human RB1.
Host
Rabbit
Theoretical MW (kDa)
102
Reactivity
Human, Mouse, Rat
Specificity
RB1 (phospho S811) polyclonal antibody detects endogenous levels of human RB1 only when phosphorylated at serine 811.
Form
Liquid
Purification
Affinity chromatography
Recommend Usage
Immunofluorescence (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (without Mg2+ and Ca2+), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: Untreated 293 cells, Lane 2: Insulin treated 293 cells, Lane 3: Untreated HeLa cells, Lane 4: UV treated HeLa cells with RB1 (phospho S811) polyclonal antibody (Cat # PAB29596) at 1:500-1:2000 dilution.Immunofluorescence
Immunofluorescent staining of U-2 OS cells with RB1 (phospho S811) polyclonal antibody (Cat # PAB29596) at 1:50-1:200 dilution. Blue: DAPI for nuclear staining. -
Gene Info — RB1
Entrez GeneID
5925Protein Accession#
P06400Gene Name
RB1
Gene Alias
OSRC, RB, p105-Rb, pRb, pp110
Gene Description
retinoblastoma 1
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq
Other Designations
OTTHUMP00000018397|retinoblastoma suspectibility protein|retinoblastoma-associated protein
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Interactome
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Pathway
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Disease
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